Nature Methods. 3D-ultrastructural imaging

Pushing borders of the nanotechnology, Peter Guttmann and colleagues developed an X-ray microscope that allows the incorporation of a cryogenic tilt stage enabling tomography of frozen-hydrated, intact cells. They were able to visualize the nuclear membrane channels and lysosomal inclusions in 3-D reconstructions of mouse adenocarcinoma cells at ~36-nm (Rayleigh) and ~70-nm (Fourier ring correlation) resolution.

Nature Methods
Published online: 14 November 2010 | doi:10.1038/nmeth.1533


17 November 2010